Supplementary Fig. 5: Lnczc3h7a promotes antiviral innate responses in mice. | Nature Immunology

Supplementary Fig. 5: Lnczc3h7a promotes antiviral innate responses in mice.

From: The long noncoding RNA Lnczc3h7a promotes a TRIM25-mediated RIG-I antiviral innate immune response

Supplementary Fig. 5

a, Schematic illustration of the knockout region of Lnczc3h7a-/- mice with two sgRNAs and test primers labeled with arrows. b, PCR analysis of genome deletion in Lnczc3h7a+/+, Lnczc3h7a+/- and Lnczc3h7a-/- mice. c, qRT-PCR analysis of Zc3h7a expression in peritoneal macrophages from Lnczc3h7a+/+and Lnczc3h7a-/- mice, infected with VSV for 8 hr. d, ELISA of IFN-α, IFN-β and IL-6 in the supernatants of peritoneal macrophages from Lnczc3h7a+/+ and Lnczc3h7a-/- mice, infected with VSV for 12 hr. e, Immunoblot analysis of RIG-I signaling pathway in peritoneal macrophages from Lnczc3h7a+/+ and Lnczc3h7a-/- mice, infected with VSV for indicated hours. f, ELISA of the production of IFN-α, IFN-β and IL-6 in sera from Lnczc3h7a+/+and Lnczc3h7a-/- mice at 24 hr after intranasal injection with influenza A virus (100 pfu per mouse). g, ELISA of the production of IFN-α, IFN-β and IL-6 in sera from Lnczc3h7a+/+and Lnczc3h7a-/- mice at 18 hr after i.v. injection with HSV-1 virus (1×107 pfu/g). h, qRT-PCR analysis of Ifna, Ifnb1, Il6 and Ifit1 mRNA levels in organs from g. i, Hematoxylin-and eosin staining of lung sections from mice in g. Scale bar, 50 μm. Data are representative of three independent experiments (b,e,i), three independent experiments with n = 3 technical replicates (c,d,f-h), each symbol represents an individual technical replicate (c,d,f-h) (shown as mean and s.e.m. in c,d,f-h), two-tailed unpaired Student’s t-test.

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