Supplementary Fig. 4: Knockdown or knockout of Lnczc3h7a blocks RIG-I-triggered antiviral signaling pathway. | Nature Immunology

Supplementary Fig. 4: Knockdown or knockout of Lnczc3h7a blocks RIG-I-triggered antiviral signaling pathway.

From: The long noncoding RNA Lnczc3h7a promotes a TRIM25-mediated RIG-I antiviral innate immune response

Supplementary Fig. 4

a, Schematic illustration of the locations of si-Lnczc3h7a target sequence. b, qRT-PCR analysis of Lnczc3h7a and Zc3h7a expression in peritoneal macrophages transfected with si-Ctrl or si-Lnczc3h7a for 48 hr and then infected with VSV for 8 hr. c, Schematic illustration of the knockout region of Lnczc3h7a-/- RAW264.7 cells with two sgRNAs and test primers labeled with arrows. d, PCR analysis of genome deletion in Lnczc3h7a-/- and Lnczc3h7a+/+ RAW264.7 cells. e, qRT-PCR analysis of Zc3h7a expression in Lnczc3h7a-/- and Lnczc3h7a+/+ RAW264.7 cells infected with VSV for 8 hr. f, Immunoblot analysis of RIG-I signaling pathway in peritoneal macrophages transfected with si- Ctrl or si-Lnczc3h7a for 48 hr and infected with VSV for indicated hours. Data are representative of three independent experiments with n = 3 technical replicates (b,e), three independent experiments (d,f), each symbol represents an individual technical replicate (b,e) (shown as mean and s.e.m. in b,e), two-tailed unpaired Student’s t-test.

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