Supplementary Fig. 3: Lnczc3h7a levels are upregulated by innate immune stimulators and are high in immune-related organs and immune cells. | Nature Immunology

Supplementary Fig. 3: Lnczc3h7a levels are upregulated by innate immune stimulators and are high in immune-related organs and immune cells.

From: The long noncoding RNA Lnczc3h7a promotes a TRIM25-mediated RIG-I antiviral innate immune response

Supplementary Fig. 3

a, qRT-PCR analysis of Lnczc3h7a and Isg54 expression in peritoneal macrophages stimulated with several types of stimulators for indicated hours. b, Northern blot analysis of Lnczc3h7a from peritoneal macrophages infected with VSV for 8 hr. c, qRT-PCR analysis of Lnczc3h7a expression in indicated organs. d, qRT-PCR analysis of Lnczc3h7a expression in indicated cells. Peritoneal macrophages were isolated from the peritoneal cavities of mice 3 days after injection with thioglycolate medium. BMDCs were isolated and induced from wild type mice’s bone marrow. CD4+ T cells, CD8+ T cells, NK cells and B cells were isolated from wild type mice’s spleens. Data are representative of three independent experiments with n = 3 technical replicates (a,c,d), three independent experiments (b), each symbol represents an individual technical replicate (a,c,d) (shown as mean and s.e.m. in a,c,d), two-tailed unpaired Student’s t-test.

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