Supplementary Figure 1: Phenotypical and functional characteristics of CD4+ T cell populations. | Nature Immunology

Supplementary Figure 1: Phenotypical and functional characteristics of CD4+ T cell populations.

From: Activation of naïve CD4+ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4+ T cells

Supplementary Figure 1

(a) Flow cytometry analysis for IFNγ, IL-4, IL-17A and IL-21 in naïve (TN) and effector memory (TEM) CD4+ T cells. Representative plot of n=3. (b) Representative histogram plots for IL-6RA (CD126), Gp130, CD62L and CD44 analysis in CD4+ TN (CD25CD44lo-intCD62LhiCD127hi), central memory (CD25CD44hiCD62LhiCD127hi) (TCM), effector (CD25CD44lo-intCD62LloCD127lo-int) (TEff), and effector memory (CD25CD44hiCD62LloCD127int-hi) (TEM) T cells. Bottom heat map shows the intensity expression of Sell (CD62L) and Il6ra genes in CD4+ TN and TEM cells. (c) Relative expression of pY-STAT1 (n=3), pY-STAT3 (n=12), pY-STAT5 (n=3), pS-STAT1 (n=3) and pS-STAT3 (n=3) in CD4+ TN, TCM, TEff and TEM cells sorted from human blood after 30 min stimulation with 20 ng/ml IL-6. **** P<0.0001, *** P<0.001 (One-way ANOVA test with Tukey multiple comparison test. Data are shown as mean ± s.e.m).

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