a) Maximum intensity projections (MIPs) of sections of popliteal LNs harvested 8 h after footpad infection with VACV-NP-S-eGFP. ERTR7 = red, rabbit polyclonal antibody against total VACV antigens = white, VACV-infected cells = green nuclear signal, B220 = blue. Scalebar = 200 μm. Boxes highlight different areas of the node in which infected cells are located. Higher magnification images are shown in the insets. Scalebars = 20 μm. For ease of viewing VACV-infected cells, the VACV-antigen-antibody signal (white) is omitted on the same inset image on the right hand side. b) As in a) but larger deconvolved images showing nuclear eGFP signal surround by white, cytoplasmic VACV Ag staining (VACV replicates in the cytoplasm). VACV eGFP signal (green) is omitted on the right. Scalebars = 20 μm. Images are representative of 6 nodes from 3 different experiments.