a, Frequency of IFN-γ+ and IFN-γ+TNF+ progenitor exhausted (Tcf1+Tim-3–) or terminally exhausted (Tcf1–Tim-3+) TILs stimulated for 6 hours ex vivo with PMA and ionomycin. Summary of one of two independent experiments, n = 10 mice. b, Representative flow plots of IL-2 production from progenitor exhausted or terminally exhausted TILs stimulated for 6 hours ex vivo with PMA and ionomycin, n = 3 independent wells. c, Representative histograms of H-2Kb expression on B16-OVA and B2m-null B16-OVA cells after 24 hours in vitro IFN-γ stimulation from one experiment. Percentage of cells within indicated gate shown. d, Schema of experimental design for in vitro killing assay. e, Representative flow plots of tumor cells after 40 hour co-culture with no T cells (left) or tetramer+ CD8+ TILs (right) stained against H-2Kb. f, Frequency of surviving target cells (B16-OVA) to control cells (B2m-null B16-OVA) normalized to no T cell wells. One of three independent experiments, n = 2 independent wells per condition. Mean +/- s.d. (a); two-sided Student’s paired t test (a); ** p ≤ 0.01.