Supplementary Figure 7: Cytokine production by fetal intestinal CD4+ T cells. | Nature Immunology

Supplementary Figure 7: Cytokine production by fetal intestinal CD4+ T cells.

From: Memory CD4+ T cells are generated in the human fetal intestine

Supplementary Figure 7

(a-c) Purified fetal intestinal CD4+ T cells were treated with a control antibody or stimulated with anti-CD3 and anti-CD28 for 4 h. Intracellular expression of TNF, IFN-γ, IL-2, IL-4 and Ki-67 was determined for each subpopulation by flow cytometry. (a) Dot plot showing the median fluorescence intensity (MFI) of TNF for each cell cluster relative to corresponding CD117+ TM subpopulation in each fetal intestine (n = 4) after stimulation with anti-CD3 and anti-CD28. Data represent two independent experiments. (b) Representative biaxial plots showing coexpression of cytokines vs. Ki-67 for the indicated cell clusters after stimulation with anti-CD3 and anti-CD28. (c) Pie charts depicting coexpression profiles of IFN-γ and IL-2 for the indicated cell clusters from fetal intestines (n = 2) after stimulation with anti-CD3 and anti-CD28. Data represent two independent experiments.

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