(a) Dot plot showing the percentage of reads per unique TCRβ sequence in each cluster. Number of unique TCRβ sequences per cluster: TN cells, 59; CD161- TM cells, 54; CD161+CD117- TM cells, 37; CD161+CD117+ TM cells, 51; Naive Treg cells, 29; Memory Treg cells, 50. (b) Hierarchical clustering of CD4+ T subpopulations based on individual V-J rearrangements in the TCRα (TRA) and TCRβ (TRB) datasets. Colors indicate different fetal intestines. (c) Dot plot showing weighted clonal overlaps for TCRβ amino acid sequences among clusters from two different fetal intestines analyzed using the F2 similarity metric in VDJtools. (d) Chord diagram showing clonal overlaps among different cell clusters. Numbers in the yellow circle indicate the total numbers and frequencies (parentheses) of private TCRβ clonotypes among TN cells. Numbers outside the plot border indicate the total numbers and frequencies (parentheses) of unique TCRβ clonotypes shared among TN cells and other CD4+ T cell subpopulations. (a) Conventional sequencing, (b-d) high-throughput sequencing.