The developmental programs that generate a broad repertoire of regulatory T cells (Treg cells) able to respond to both self antigens and non-self antigens remain unclear. Here we found that mature Treg cells were generated through two distinct developmental programs involving CD25+ Treg cell progenitors (CD25+ TregP cells) and Foxp3lo Treg cell progenitors (Foxp3lo TregP cells). CD25+ TregP cells showed higher rates of apoptosis and interacted with thymic self antigens with higher affinity than did Foxp3lo TregP cells, and had a T cell antigen receptor repertoire and transcriptome distinct from that of Foxp3lo TregP cells. The development of both CD25+ TregP cells and Foxp3lo TregP cells was controlled by distinct signaling pathways and enhancers. Transcriptomics and histocytometric data suggested that CD25+ TregP cells and Foxp3lo TregP cells arose by coopting negative-selection programs and positive-selection programs, respectively. Treg cells derived from CD25+ TregP cells, but not those derived from Foxp3lo TregP cells, prevented experimental autoimmune encephalitis. Our findings indicate that Treg cells arise through two distinct developmental programs that are both required for a comprehensive Treg cell repertoire capable of establishing immunotolerance.
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The data that support the findings of this study are available from the corresponding author upon request. Single-cell RNA-seq data were deposited at Gene Expression Omnibus, with the following accession code: GSE123067.
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We thank G. Hubbard, A. Rost, A. Meskic, D. Duerre and H. Wiesolek for technical assistance; T. Martin, N. Shah, J. Motl and P. Champoux for cell sorting and maintenance of the Flow Cytometry Core Facility at the University of Minnesota (5P01AI035296); S. Hamilton, M. Pierson and funding from the University of Minnesota academic health center for maintaining the NME mouse facility; P. Fink for providing initial Rag2-GFP thymi; B. Burbach and Y. Shimizu for Adap–/– mice; M. Jenkins for the MOG:I-Ab tetramer; and C. Katerndahl and L. Heltemes-Harris for helpful commentary and reviewing the manuscript. D.L.O. and S.A.M. were supported by an immunology training grant (no. 2T32AI007313). S.A.M. was also supported by an individual predoctoral F30 fellowship from the National Institutes of Health (NIH; no. F30DK096844). J.A.S. was supported by University of Minnesota Medical Foundation grant no. UMF0020624 and NIH grants nos 5U24AI118635 and R01AI106791. Y.Z. was supported by NIH grant no. R01AI107027. U.B. and C.B.W. were supported by grants from the Children’s Hospital of Wisconsin, and C.B.W. was also supported by NIH grant no. R01AI085090-07A1. A.M. and M.S.A were supported by NIH grant no. DP3DK111914-01. A.M. holds a Career Award for Medical Scientists from the Burroughs Wellcome Fund and is an investigator at the Chan Zuckerberg Biohub. M.A. was supported by NIH grant no. R01AI115716. M.S.A. was supported by NIH grant no. R37 AI097457. A.A. was supported by NIH grants nos AI108958, AI120701, AI126814 and AI129422 to A.A. and W.H. W.H. was supported by NIH grant no. AI29422 (to W.H. and A.A.), a Careers in Immunology Fellowship from the American Association of Immunologists, a Faculty Development Award and a competitive research grant from Louisiana State University, and a pilot award from the LSU-Tulane Center for Experimental Infectious Diseases Research funded by NIH grant no. GM110760. M.A.F. was supported by NIH grants nos AI124512, AI113138, AI061165, CA154998, CA151845 and CA185062.
A.M. is a co-founder of Spotlight Therapeutics. A.M. has served as an advisor to Juno Therapeutics and is a member of the scientific advisory board at PACT Pharma. The Marson laboratory has received sponsored research support from Juno Therapeutics, Epinomics and Sanofi, and a gift from Gilead. A.A. has received sponsored research support from 3 M. MAF has received sponsored research support from Merck.
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Integrated supplementary information
Sorted Treg cell lineage subsets were stimulated for 3 days in the indicated concentrations of IL-2 and analyzed for the % of cells which converted into (left and middle panel) or remained (right panel) CD25+Foxp3+ mature Treg cells. Data represents 1 experiment, n = 2 (CD25+ TregP cells- untreated, 0.2 Uml–1 IL2; Foxp3lo TregP cells- untreated, 100 Uml–1 IL-2; Treg cells- 0.04 Uml–1 IL-2, 0.2 Uml–1 IL-2, 1 Uml–1 IL-2), n = 1 (Foxp3lo TregP cells- 0.04 Uml–1 IL-2; Treg cells- untreated), n = 3 (CD25+ TregP cells- 0.04 Uml–1 IL-2, 1 Uml–1 IL-2, 100 Uml–1 IL-2; Foxp3lo TregP cells- 0.2 Uml–1 IL-2, 1 Uml–1 IL-2; Treg cells- 100 Uml–1 IL-2) technical replicates. Bars represent mean ± SD.
a) Representative flow plots of SPF, germ-free reconstituted and germ-free mice thymi and quantification of the percent of each Treg cell lineage subset within CD4+CD73- thymocytes. b) Representative flow plots and quantification of SPF, germ-free reconstituted and germ free mice spleens showing the percent of CD4+ lymphocytes which are Foxp3+. a,b) Data is representative of 1 experiment, n = 6 SPF mice, 5 germ-free reconstituted mice, and 6 germ-free mice. Data was analyzed by one-way ANOVA with Tukey’s multiple comparisons test. c) Representative flow plots of CD4+CD73- thymocytes from SPF mice or mice with normalized microbial experience (NME) and quantification of the percent within each Treg cell lineage population. Data is representative of 2 experiments, n = 3 SPF mice and 5 NME mice. Data was analyzed by two-sided unpaired t test. All bars represent mean ± SD. *P<0.05, **P<0.005, ns- not significant.
a) Violin plots (left) or feature plots (right) displaying single-cell expression for either Foxp3-GFP reporter (top) or Il2ra (bottom) for each cluster from the single-cell RNA-seq data set presented in Fig. 2e,f. b) Data from an independent repeat of 10x Genomics single-cell RNA-seq. Heatmap displays the top 10 differentially regulated genes in each cluster from the data set presented in Fig. 2e,f. c) Violin plots displaying single-cell expression for Bcl2l11 (left) and Nr4a1 (right) for each cluster from the single-cell RNA-seq data set presenting in Fig. 2e,f. a-c) Data is representative from 3 independent experiments, n = 3 mice.
a) Thymocytes of the indicated subsets were analyzed for expression of HSA and Qa-2. Gates are drawn to demonstrate the frequency of cells within each maturation state. Data is representative of 1 experiment, n = 2 mice. b) Thymocytes of the indicated subsets were analyzed for CD69 and MHC-I expression. Gates are drawn to demonstrate the frequency of cells within each maturation state. Data is representative of 1 experiment, n = 3 mice.
Supplementary Figure 5 Frequency of contaminating recirculating cells in thymic Treg cell lineage subsets.
Thymocytes of the indicated subsets were analyzed for RAG2-GFP expression. Gates were drawn to determine the frequency of RAG2-GFP- (recirculating) and RAG2-GFP+ (newly developing) fractions of cells within each subset. Displayed are concatenated data from 3 thymi. Results are representative of 7 experiments, n = 9 mice.
a) Quantification of Foxp3-gMFI in mature, CD73+ thymic Treg cells in WT, Foxp3-GFPKIN or Foxp3 Cns3-/- mice. All data points are normalized to the Foxp3-GFPKIN average within each experiment. Data is representative of 4 experiments, n = 8 wild-type mice, 14 GFPKIN mice and 14 Cns3-/- mice. Data was analyzed by a one-way ANOVA with Tukey’s multiple comparisons test. b) Quantification of CD25-gMFI in CD73- thymic Treg cells in WT or EDEL (Il2ra CaRE4-/-) mice in the non-obese diabetic (NOD) background. Data is representative of 3 experiments, n = 10 wild-type NOD mice and 10 EDEL NOD mice. Data was analyzed by a two-sided Mann-Whitney test. a,b) Bars represent mean ± SD. *P<0.05, ns- not significant.
a,b) Flow plots of the indicated TregP cell subsets following 3 days of stimulation with the indicated cytokines. c,d) Quantification of the gMFI of CD25 or Foxp3 within mature Treg cells (CD25+Foxp3+) generated from the indicated cytokine conditions. Data was analyzed by two-sided Kruskal-Wallis test. Data represents 3 experiments, n = 9 (CD25+ TregP cells- 1 Uml–1 IL2, 1 ng/mL IL4), n = 8 (CD25+ TregP cells- 100 ng/mL IL4, 1 Uml–1 IL2 + 1 ng/mL IL4, 1 Uml–1 IL2 + 100 ng/mL IL4; Foxp3lo TregP cells- 1 Uml–1 IL2, 1 Uml–1 IL2 + 1 ng/mL IL4, 1 Uml–1 IL2 + 100 ng/mL IL4), n = 7 (Foxp3lo TregP cells- 1 ng/mL IL4, 100 ng/mL IL4) or 1 experiment, n = 3 (CD25+ TregP cells- 0.5 ng/mL IL4, 1 Uml–1 IL2 + 0.5 ng/mL IL4; Foxp3lo TregP cells- 1 Uml–1 IL2 + 0.5 ng/mL IL4), n = 2 (Foxp3lo TregP cells- 0.5 ng/mL IL4) replicates. Bars represent mean ± SD. *P<0.05; **P<0.005; ***P<0.0001.
Supplementary Figure 8 Treg cells derived from Foxp3lo TregP cells protect against transfer induced colitis.
Data depicts % starting weight from Foxp3lo TregP cell transfer over the time-course of colitis experiment. Graph represents 2 experiments, n = 4 mice per group. Data was analyzed by two-sided multiple t test with Holm-Sidak method. *adjusted p value<0.05. Bars represent mean ± SEM.
Supplementary Table 1 and Supplementary Note
Differentially expressed genes between CD25+ TregP and Foxp3lo TregP cells from single-cell RNA-seq data sets. This table displays differentially expressed genes between CD25+ and Foxp3lo TregP cells in 2 independent single-cell RNA-seq data sets. The list is annotated by protein type and if it is a known gene correlated with negative selection
The video displays expression of CD25 and Foxp3 for cells falling within different bins of RAG2-GFP expression, from high RAG2-GFP to RAG2-GFP low (RAG2-GFP negative cells were excluded here). Data is from 3 concatenated thymi and is representative of 6 additional independent experiments
Code used to analyze single cell RNA-seq data
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Owen, D.L., Mahmud, S.A., Sjaastad, L.E. et al. Thymic regulatory T cells arise via two distinct developmental programs. Nat Immunol 20, 195–205 (2019). https://doi.org/10.1038/s41590-018-0289-6
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