Supplementary Figure 1: Conversion of distinct TregP cell subsets into mature Treg cells in vitro. | Nature Immunology

Supplementary Figure 1: Conversion of distinct TregP cell subsets into mature Treg cells in vitro.

From: Thymic regulatory T cells arise via two distinct developmental programs

Supplementary Figure 1

Sorted Treg cell lineage subsets were stimulated for 3 days in the indicated concentrations of IL-2 and analyzed for the % of cells which converted into (left and middle panel) or remained (right panel) CD25+Foxp3+ mature Treg cells. Data represents 1 experiment, n = 2 (CD25+ TregP cells- untreated, 0.2 Uml–1 IL2; Foxp3lo TregP cells- untreated, 100 Uml–1 IL-2; Treg cells- 0.04 Uml–1 IL-2, 0.2 Uml–1 IL-2, 1 Uml–1 IL-2), n = 1 (Foxp3lo TregP cells- 0.04 Uml–1 IL-2; Treg cells- untreated), n = 3 (CD25+ TregP cells- 0.04 Uml–1 IL-2, 1 Uml–1 IL-2, 100 Uml–1 IL-2; Foxp3lo TregP cells- 0.2 Uml–1 IL-2, 1 Uml–1 IL-2; Treg cells- 100 Uml–1 IL-2) technical replicates. Bars represent mean ± SD.

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