a) Quantification of Foxp3-gMFI in mature, CD73+ thymic Treg cells in WT, Foxp3-GFPKIN or Foxp3 Cns3-/- mice. All data points are normalized to the Foxp3-GFPKIN average within each experiment. Data is representative of 4 experiments, n = 8 wild-type mice, 14 GFPKIN mice and 14 Cns3-/- mice. Data was analyzed by a one-way ANOVA with Tukey’s multiple comparisons test. b) Quantification of CD25-gMFI in CD73- thymic Treg cells in WT or EDEL (Il2ra CaRE4-/-) mice in the non-obese diabetic (NOD) background. Data is representative of 3 experiments, n = 10 wild-type NOD mice and 10 EDEL NOD mice. Data was analyzed by a two-sided Mann-Whitney test. a,b) Bars represent mean ± SD. *P<0.05, ns- not significant.