Supplementary Figure 2: Baseline immune response of wild-type and Pik3cdE1020K/+ mice in lymph node and spleen. | Nature Immunology

Supplementary Figure 2: Baseline immune response of wild-type and Pik3cdE1020K/+ mice in lymph node and spleen.

From: Hyperactivated PI3Kδ promotes self and commensal reactivity at the expense of optimal humoral immunity

Supplementary Figure 2

a, Representative flow plots and histogram of BCL-6 and CXCR5 staining for GC-TFH, pre-TFH and non-TFH cells (gated on CD4+B220 T cells in the spleen) (n=6 per group). b, Frequency of PD-1+CXCR5+Foxp3 TFH cells (percent of CD4+B220 T cells) in the popliteal lymph node of 2 and 4-month-old mice at steady state. c, Representative flow plots and cell counts of CD4+ follicular T cells: CXCR5+PD-1+ (left panel) and CXCR5+ICOS+ (right panel) in 1-year-old mice in the spleen. d, Frequency of GL-7+FAS+ GC B cells (percent of B220+CD19+ B cells) in the popliteal lymph node of 2 and 4-month-old mice at steady state (b,d, 2 month-old, n=5 per group; 4 month-old, wild-type n=8, Pik3cdE1020K/+ n=7). e, Representative flow plots and cell counts of GL-7+FAS+ GC B cells (percent of B220+CD19+ B cells, left panel), IgD+ B cells (percent of B220+CD19+ B cells, right panel), and CD138+B220int/lo plasma cells (of live cells, lower panel) in 1-year-old mice in the spleen (c, e, wild-type n=3, Pik3cdE1020K/+ n=5). Data are representative of two independent experiments. Data are represented as mean ± SEM. Significance analyzed by Mann-Whitney U test. *P < 0.05; **P < 0.01; ***P < 0.001