Cytometry A. https://doi.org/10.1002/cyto.a.23331 (2018)

High-dimensional analysis of immune cells is becoming increasingly prevalent in immunology and can provide in-depth information on rare or transitional populations; however, it can be technically challenging and expensive and can require the use of highly specialized reagents. In Cytometry A, Mair and Prlic describe a conventional flow-cytometry staining panel that incorporates 28 colors plus two physical parameters (forward scatter and side scatter). The panel covers all canonical human dendritic-cell subsets but also allows parallel analysis of monocytes and lymphocytes. The 30 parameters presented enable the description of human cells by flow cytometry in unparalleled depth without the need for ‘bespoke’ equipment or reagents and approach the dimensionality of more-specialized technologies such as mass cytometry.