Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Spatial proteomics

Illuminating GPCR trafficking

Nature Chemical Biology (2024)Cite this article

Subjects

A method to study G-protein-coupled receptor (GPCR) trafficking has been developed using engineered APEX2 and CRISPR interference screening. The innovative approach reveals a network of proteins coordinated by DNAJC13 that control efficient GPCR sorting into degradative or recycling pathways.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Regulation of GPCR trafficking at the endosome.

References

  1. Irannejad, R. & Lobingier, B. T. in GPCRs as Therapeutic Targets (ed. Gilchrist, A.) Vol. 2, 180–196 (John Wiley and Sons, 2022).

  2. Novy, B. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-024-01705-2 (2024).

    Article  PubMed  PubMed Central  Google Scholar 

  3. Milo, R. Bioessays 35, 1050–1055 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Qin, W., Cho, K. F., Cavanagh, P. E. & Ting, A. Y. Nat. Methods 18, 133–143 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Gilbert, L. A. et al. Cell 159, 647–661 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Lam, S. S. et al. Nat. Methods 12, 51–54 (2015).

    Article  CAS  PubMed  Google Scholar 

  7. Klauer, M. J., Willette, B. K. A. & Tsvetanova, N. G. J. Biol. Chem. 300, 105668 (2024).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. von Zastrow, M. & Sorkin, A. Annu. Rev. Biochem. 90, 709–737 (2021).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Drug Discovery Biology Theme, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, Australia

    Michelle L. Halls

Authors
  1. Michelle L. Halls
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michelle L. Halls.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Halls, M.L. Illuminating GPCR trafficking. Nat Chem Biol (2024). https://doi.org/10.1038/s41589-024-01699-x

Download citation

  • Published:

  • DOI: https://doi.org/10.1038/s41589-024-01699-x

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing