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LIS1 (Pac1) binding slows dissociation of dynein from microtubules

LIS1 is an essential cofactor for the assembly of the cytoplasmic dynein transport machinery. How LIS1 binding affects dynein motility was unclear. Single-molecule experiments reveal that Pac1 (the yeast homolog of LIS1) binding reduces dynein speed by slowing its detachment from microtubules and does not disrupt the mechanism by which it generates force.

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Fig. 1: Lis1 binding alters the microtubule-affinity and velocity of dynein.


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This is a summary of: Kusakci, E. et al. Lis1 slows force-induced detachment of cytoplasmic dynein from microtubules. Nat. Chem. Biol. (2023).

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LIS1 (Pac1) binding slows dissociation of dynein from microtubules. Nat Chem Biol (2023).

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