Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Proteomics

Proteoforms feel the heat

Identifying new proteoforms — structural variants of proteins — is frequently challenging, particularly on the proteome-wide scale. A new study leverages their differential thermal stabilities to identify proteoform functional groups by deep thermal proteome profiling.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Structural variations of proteoforms influence thermal stability.

References

  1. Smith, L. M. et al. Nat. Methods 10, 186–187 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Kurzawa, N. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-023-01284-8 (2023).

    Article  PubMed  Google Scholar 

  3. Toby, T. K., Fornelli, L. & Kelleher, N. L. Annu. Rev. Anal. Chem. 9, 499–519 (2016).

    Article  CAS  Google Scholar 

  4. Dermit, M., Peters-Clarke, T. M., Shishkova, E. & Meyer, J. G. J. Proteome Res. 20, 1972–1980 (2021).

    Article  CAS  PubMed  Google Scholar 

  5. Bludau, I. et al. Nat. Commun. 12, 3810 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Martinez Molina, D. et al. Science 341, 84–87 (2013).

    Article  PubMed  Google Scholar 

  7. Mateus, A. et al. Thermal proteome profiling for interrogating protein interactions. Mol. Syst. Biol. 16, e9232 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Kelleher, N. L., Thomas, P. M., Ntai, I., Compton, P. D. & LeDuc, R. D. Expert Rev. Proteomics 11, 649–651 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Leo, I. R. et al. Nat. Commun. 13, 1691 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Smith, L. M. et al. Nat. Methods 16, 939–940 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Bryon S. Drown.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Campbell, T.L., Drown, B.S. Proteoforms feel the heat. Nat Chem Biol 19, 930–931 (2023). https://doi.org/10.1038/s41589-023-01285-7

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41589-023-01285-7

Search

Quick links

Nature Briefing: Cancer

Sign up for the Nature Briefing: Cancer newsletter — what matters in cancer research, free to your inbox weekly.

Get what matters in cancer research, free to your inbox weekly. Sign up for Nature Briefing: Cancer