This is a preview of subscription content, access via your institution
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Rent or buy this article
Prices vary by article type
Prices may be subject to local taxes which are calculated during checkout
Kim, D. Y. et al. Hypercompact adenine base editors based on transposase B guided by engineered RNA. Nat. Chem. Biol. https://doi.org/10.1038/s41589-022-01077-5 (2022).
Karvelis, T. et al. Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease. Nature 599, 692–696 (2021).
Altae-Tran, H. et al. The widespread IS200/605 transposon family encodes diverse programmable RNA-guided endonucleases. Science https://doi.org/10.1126/science.abj6856 (2021).
He, S. et al. The IS200/IS605 family and ‘peel and paste’ single-strand transposition mechanism. Microbiol. Spectr. https://doi.org/10.1128/microbiolspec.MDNA3-0039-2014 (2015).
Wang, J. Y., Pausch, P. & Doudna, J. A. Structural biology of CRISPR-Cas immunity and genome editing enzymes. Nat. Rev. Microbiol. 20, 641–656 (2022).
Kim, D. Y. et al. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat. Biotechnol. https://doi.org/10.1038/s41587-021-01009-z (2021).
Wu, Z. et al. Programmed genome editing by a miniature CRISPR-Cas12f nuclease. Nat. Chem. Biol. https://doi.org/10.1038/s41589-021-00868-6 (2021).
Xu, X. et al. Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing. Mol. Cell https://doi.org/10.1016/j.molcel.2021.08.008 (2021).
Bigelyte, G. et al. Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells. Nat. Commun. 12, 6191 (2021).
Harrington, L. B. et al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science 362, 839–842 (2018).
Karvelis, T. et al. PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage. Nucleic Acids Res. 48, 5016–5023 (2020).
Altschul, S. F. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402 (1997).
Makarova, K. S. et al. Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants. Nat. Rev. Microbiol. 18, 67–83 (2020).
T.K. and V.S. are co-inventors on a patent application (PCT/IB2021/055958) filed by Vilnius University. V.S. is a chairman of and has financial interest in CasZyme.
Peer review information
Nature Chemical Biology thanks the anonymous reviewers for their contribution to the peer review of this work.
About this article
Cite this article
Karvelis, T., Siksnys, V. Mis-annotation of TnpB: case of TaRGET-ABE. Nat Chem Biol 19, 261–262 (2023). https://doi.org/10.1038/s41589-022-01242-w
This article is cited by
Author Correction: Hypercompact adenine base editors based on a Cas12f variant guided by engineered RNA
Nature Chemical Biology (2023)