Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Membrane transporters

Deep dive into a drug pump

Major hurdles remain in understanding the mechanisms of multidrug resistance (MDR) protein efflux. A new study uses deep mutational scanning of a bacterial MDR protein to determine the nature of its drug-binding cavity and understand its function and plasticity.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Fig. 1: Flowchart showing steps of DMS analysis of EfrCD.

References

  1. Chufan, E. E., Sim, H. M. & Ambudkar, S. V. Adv. Cancer Res. 125, 71–96 (2015).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Du, D. et al. Nat. Rev. Microbiol. 16, 523–539 (2018).

    Article  CAS  PubMed  Google Scholar 

  3. Kaur, P. Drug Resist. Updat. 5, 61–64 (2002).

    Article  PubMed  Google Scholar 

  4. Meier, G. Nat. Chem. Biol. https://doi.org/10.1038/s41589-022-01205-1 (2022).

  5. Fowler, D. M. & Fields, S. Nat. Methods 11, 801–807 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Aller, S. G. et al. Science 323, 1718–1722 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Brown, K., Li, W. & Kaur, P. Biochemistry 56, 1921–1931 (2017).

    Article  CAS  PubMed  Google Scholar 

  8. Loo, T. W. & Clarke, D. M. J. Biol. Chem. 275, 39272–39278 (2000).

    Article  CAS  PubMed  Google Scholar 

  9. Schuster, S. et al. Antimicrob. Agents Chemother. 58, 6870–6878 (2014).

    Article  PubMed  PubMed Central  Google Scholar 

  10. Esser, L. et al. J. Biol. Chem. 292, 446–461 (2017).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Parjit Kaur.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kaur, P. Deep dive into a drug pump. Nat Chem Biol (2022). https://doi.org/10.1038/s41589-022-01201-5

Download citation

  • Published:

  • DOI: https://doi.org/10.1038/s41589-022-01201-5

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing