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Single-molecule biophysics

Very fast nucleotides on demand

Single-molecule methods are a powerful tool to study the kinetics of ATP-powered enzymes. A method that locally controls the generation of ATP significantly increases the throughput of single-molecule approaches and unlocks single-turnover analysis of molecular machines.

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Fig. 1: Local generation of NTPs by photolysis of caged nucleotides.


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Correspondence to Jens C. Schmidt.

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Schmidt, J.C. Very fast nucleotides on demand. Nat Chem Biol 18, 1043–1044 (2022).

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