Single-molecule methods are a powerful tool to study the kinetics of ATP-powered enzymes. A method that locally controls the generation of ATP significantly increases the throughput of single-molecule approaches and unlocks single-turnover analysis of molecular machines.
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Schmidt, J.C. Very fast nucleotides on demand. Nat Chem Biol 18, 1043–1044 (2022). https://doi.org/10.1038/s41589-022-01144-x
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DOI: https://doi.org/10.1038/s41589-022-01144-x