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SYNTHETIC BIOLOGY

Shackled ribosomes unleashed

A directed evolution approach delivers ribosomes with highly functional tethered subunits. Combining the decoding and peptidyl transferase activities of the ribosome into a single entity sets the scene for more efficient protein engineering technologies.

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Fig. 1: Engineering of efficient tethered ribosomes via evolution and linkage (EvoLink).

References

  1. Crick, F. H. J. Mol. Biol. 38, 367–379 (1968).

    CAS  Article  Google Scholar 

  2. de la Torre, D. & Chin, J. W. Nat. Rev. Genet. 22, 169–184 (2021).

    Article  Google Scholar 

  3. Kim, D. S. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-022-01064-w (2022).

  4. Orelle, C., Carlson, E. D., Szal, T., Florin, T., Jewett, M. C. & Mankin, A. S. Nature. 524, 119–124 (2015).

    CAS  Article  Google Scholar 

  5. Fried, S. D., Schmied, W. H., Uttamapinant, C. & Chin, J. W. Angew. Chem. 127, 12982–12985 (2015).

    Article  Google Scholar 

  6. Schmied, W. H., Tnimov, Z., Uttamapinant, C., Rae, C. D., Fried, S. D. & Chin, J. W. Nature 564, 444–448 (2018).

    CAS  Article  Google Scholar 

  7. Carlson, E. D. et al. Nat. Commun. 10, 3920 (2019).

    Article  Google Scholar 

  8. Rackham, O. & Chin, J. W. Nat. Chem. Biol. 1, 159–166 (2005).

    CAS  Article  Google Scholar 

  9. Filipovska, A. & Rackham, O. FEBS Lett. 587, 1189–1197 (2013).

    CAS  Article  Google Scholar 

  10. Fabret, C. & Namy, O. Nucleic Acids Res. 49, 5308–5318 (2021).

    CAS  Article  Google Scholar 

Download references

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Correspondence to Oliver Rackham.

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Filipovska, A., Rackham, O. Shackled ribosomes unleashed. Nat Chem Biol 18, 918–920 (2022). https://doi.org/10.1038/s41589-022-01020-8

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  • DOI: https://doi.org/10.1038/s41589-022-01020-8

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