Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.


Activity-based profiling in RNA

RNA modifications are emerging regulators of development and disease. A metabolic labeling approach using 5-fluorocytidine (5-FCyd) allows the mechanism-based profiling of several RNA-modifying enzymes and potentially links their activity to novel RNA targets.

This is a preview of subscription content

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Fig. 1: Metabolic labeling of RNA by 5-FCyd enables activity-based profiling of RNA-modifying enzymes.


  1. 1.

    Saghatelian, A. & Cravatt, B. F. Nat. Chem. Biol. 1, 130–142 (2005).

    CAS  Article  Google Scholar 

  2. 2.

    Liu, Y., Patricelli, M. P. & Cravatt, B. F. Proc. Natl. Acad. Sci. USA 96, 14694–14699 (1999).

    CAS  Article  Google Scholar 

  3. 3.

    Dai, W. et al. Nat. Chem. Biol. (2021).

  4. 4.

    Wiener, D. & Schwartz, S. Nat. Rev. Genet. 22, 119–131 (2021).

    CAS  Article  Google Scholar 

  5. 5.

    Khoddami, V. & Cairns, B. R. Nat. Biotechnol. 31, 458–464 (2013).

    CAS  Article  Google Scholar 

  6. 6.

    Anders, L. et al. Nat. Biotechnol. 32, 92–96 (2014).

    CAS  Article  Google Scholar 

  7. 7.

    Bantscheff, M. et al. Nat. Biotechnol. 25, 1035–1044 (2007).

    CAS  Article  Google Scholar 

  8. 8.

    Montgomery, D. C., Sorum, A. W. & Meier, J. L. J. Am. Chem. Soc. 136, 8669–8676 (2014).

    CAS  Article  Google Scholar 

  9. 9.

    Selmi, T. et al. Nucleic Acids Res. 49, 1006–1022 (2021).

    CAS  Article  Google Scholar 

  10. 10.

    Marchand, V., Ayadi, L., Bourguignon-Igel, V., Helm, M. & Motorin, Y. Methods Mol. Biol. 2298, 77–95 (2021).

    Article  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Jordan L. Meier.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Thalalla Gamage, S., Meier, J.L. Activity-based profiling in RNA. Nat Chem Biol 17, 1121–1122 (2021).

Download citation


Quick links