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A new phase for enzyme kinetics

Liquid–liquid phase separation can increase the rate of enzyme activity by concentrating reactants together. A phase-separating SUMOylation cascade offers conceptual and quantitative insight into the mechanisms underlying the activity enhancement.

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Fig. 1: Liquid–liquid phase separation can function to increase enzyme activity.


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T.M. acknowledges funding by NIH grant R01GM112846, by the St. Jude Children’s Research Hospital Research Collaborative on Membrane-less Organelles in Health and Disease, and by the American Lebanese Syrian Associated Charities (ALSAC). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Correspondence to Tanja Mittag.

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Competing interests

T.M. is a consultant for Faze Medicines. This affiliation has not influenced the scientific content of this piece.

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O’Flynn, B.G., Mittag, T. A new phase for enzyme kinetics. Nat Chem Biol 17, 628–630 (2021).

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