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Expanding the membrane-protein NMR toolkit

An NMR method to monitor conformational states of challenging large protein targets is described. The method, which can be used to evaluate distances between two labels and to measure conformational exchange rates, revealed an unanticipated outward-facing state in a glutamate transporter.

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Fig. 1: 19F PRE NMR as a complement to FRET for evaluating conformational states.

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Correspondence to Merritt Maduke.

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Cheng, R.C., Maduke, M. Expanding the membrane-protein NMR toolkit. Nat Chem Biol 16, 937–938 (2020). https://doi.org/10.1038/s41589-020-0597-7

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