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Expanding the membrane-protein NMR toolkit

Nature Chemical Biology volume 16, pages 937–938 (2020)Cite this article

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An NMR method to monitor conformational states of challenging large protein targets is described. The method, which can be used to evaluate distances between two labels and to measure conformational exchange rates, revealed an unanticipated outward-facing state in a glutamate transporter.

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Fig. 1: 19F PRE NMR as a complement to FRET for evaluating conformational states.

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Authors and Affiliations

  1. Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA

    Ricky C. Cheng & Merritt Maduke

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  1. Ricky C. Cheng
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  2. Merritt Maduke
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Correspondence to Merritt Maduke.

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The authors declare no competing interests.

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Cheng, R.C., Maduke, M. Expanding the membrane-protein NMR toolkit. Nat Chem Biol 16, 937–938 (2020). https://doi.org/10.1038/s41589-020-0597-7

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