CHEMOPROTEOMICS

Sweetly profiling the cysteinome

A new sugar-based cysteine-reactive probe, combined with competitive activity-based protein profiling (ABPP), enables site-centric target deconvolution of itaconate in native proteomes, shedding light on a novel mechanism of action for this important immunoregulatory metabolite in inflammatory macrophages.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: Chemoproteomics reveals a novel mechanism of action of itaconate in macrophages.

References

  1. 1.

    Mills, E. L. et al. Nature 556, 113–117 (2018).

  2. 2.

    Bambouskova, M. et al. Nature 556, 501–504 (2018).

  3. 3.

    Qin, W. et al. Nat. Chem. Biol. https://doi.org/10.1038/10.1038/s41589-019-0323-5 (2019).

  4. 4.

    Backus, K. M. et al. Nature 534, 570–574 (2016).

  5. 5.

    Qin, W. et al. Angew. Chem. Int. Edn Engl. 57, 1817–1820 (2018).

  6. 6.

    Qin, K. et al. ACS Chem. Biol. 13, 1983–1989 (2018).

  7. 7.

    Puchalska, P. et al. iScience 9, 298–313 (2018).

  8. 8.

    Van den Bossche, J., O’Neill, L. A. & Menon, D. Trends Immunol. 38, 395–406 (2017).

  9. 9.

    Mills, E. L. et al. Cell 167, 457–470.e13 (2016).

  10. 10.

    Yang, J., Tallman, K. A., Porter, N. A. & Liebler, D. C. Anal. Chem. 87, 2535–2541 (2015).

  11. 11.

    Yang, J., Gupta, V., Carroll, K. S. & Liebler, D. C. Nat. Commun. 5, 4776 (2014).

Download references

Author information

Correspondence to Jing Yang.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and Permissions

About this article