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An excreted small molecule promotes C. elegans reproductive development and aging

Nature Chemical Biology volume 15, pages 838–845 (2019)Cite this article

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Abstract

Excreted small-molecule signals can bias developmental trajectories and physiology in diverse animal species. However, the chemical identity of these signals remains largely obscure. Here we report identification of an unusual N-acylated glutamine derivative, nacq#1, that accelerates reproductive development and shortens lifespan in Caenorhabditis elegans. Produced predominantly by C. elegans males, nacq#1 hastens onset of sexual maturity in hermaphrodites by promoting exit from the larval dauer diapause and by accelerating late larval development. Even at picomolar concentrations, nacq#1 shortens hermaphrodite lifespan, suggesting a trade-off between reproductive investment and longevity. Acceleration of development by nacq#1 requires chemosensation and is dependent on three homologs of vertebrate steroid hormone receptors. Unlike ascaroside pheromones, which are restricted to nematodes, fatty acylated amino acid derivatives similar to nacq#1 have been reported from humans and invertebrates, suggesting that related compounds may serve signaling functions throughout metazoa.

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Fig. 1: Excreted small molecules accelerate development in C. elegans.
Fig. 2: Identification of nacq#1, a signaling molecule primarily produced by males.
Fig. 3: Biological properties of nacq#1.
Fig. 4: nacq#1 and ascarosides are mutually antagonistic signals.
Fig. 5: nacq#1 signals via conserved signaling pathways.

Data availability

The authors declare that the data supporting the findings of this study are available within the article and its supplementary information files.

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Acknowledgements

This work was supported in part by National Institutes of Health grants R01GM113692 (to F.C.S.), R01GM088290 (to F.C.S.) and T32GM008500 (to R.N.B.), R01GM126125 (to I.R.), and by National Science Foundation (NSF) grants IOS-1708518 and IOS-1755244 (to I.R.). F.C.S. is a faculty scholar of the Howard Hughes Medical Institute. This work made use of the Cornell University NMR Facility, which is supported, in part, by the NSF through MRI award CHE-1531632. We thank N. Movahed and D. Kiemle for assistance with MS and NMR spectroscopy and R. Smith and G. Horvath for technical support.

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Authors and Affiliations

  1. Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, USA

    Andreas H. Ludewig, Alexander B. Artyukhin, Pedro R. Rodrigues, Dania C. Pulido, Russell N. Burkhardt, Oishika Panda, Ying K. Zhang, Pooja Gudibanda & Frank C. Schroeder

  2. Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA

    Erin Z. Aprison & Ilya Ruvinsky

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Contributions

I.R. and F.C.S. supervised the study. A.H.L., A.B.A., I.R. and F.C.S. designed experiments. A.H.L., A.B.A., E.Z.A., P.R.R., D.C.P., R.N.B., P.G. and O.P. performed chemical and biological experiments. R.N.B. and Y.K.Z. performed syntheses. I.R. and F.C.S. wrote the paper with input from the other authors.

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Correspondence to Ilya Ruvinsky or Frank C. Schroeder.

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Supplementary information

Supplementary Information

Supplementary Tables 1–15 and Supplementary Figures 1–14

Reporting Summary

Supplementary Note

Synthetic procedures

Supplementary Data Set

Data for time-of-development assays based on morphological criteria. Includes data for time-of-development assays and molting curves shown in Figs. 1b and 3b,d, and Supplementary Figs. 5b, 7b,c, 9 and 12b,c.

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Ludewig, A.H., Artyukhin, A.B., Aprison, E.Z. et al. An excreted small molecule promotes C. elegans reproductive development and aging. Nat Chem Biol 15, 838–845 (2019). https://doi.org/10.1038/s41589-019-0321-7

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