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PEPTIDE LIBRARIES

Affinity selection and sequencing

Powerful combinatorial peptide library methods allow the discovery of peptide leads from diverse libraries. A new platform based on tandem mass spectrometry peptide sequencing coupled with high-performance size-exclusion chromatography enables identification of high-affinity peptidic ligands from focused libraries.

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References

  1. 1.

    Liu, R., Li, X. & Lam, K. S. Curr. Opin. Chem. Biol. 38, 117–126 (2017).

  2. 2.

    Scott, J. K. & Smith, G. P. Science 249, 386–390 (1990).

  3. 3.

    Lam, K. S. et al. Nature 354, 82–84 (1991).

  4. 4.

    Houghten, R. A. et al. Nature 354, 84–86 (1991).

  5. 5.

    Josephson, K., Hartman, M. C. & Szostak, J. W. J. Am. Chem. Soc. 127, 11727–11735 (2005).

  6. 6.

    Heinis, C., Rutherford, T., Freund, S. & Winter, G. Nat. Chem. Biol. 5, 502–507 (2009).

  7. 7.

    Franzini, R. M., Neri, D. & Scheuermann, J. Acc. Chem. Res. 47, 1247–1255 (2014).

  8. 8.

    Touti, F. et al. Nat. Chem. Biol. https://doi.org/10.1038/S41589-019-0245-2 (2019).

  9. 9.

    Furka, A., Sebestyén, F., Asgedom, M. & Dibó, G. Int. J. Pept. Protein Res. 37, 487–493 (1991).

  10. 10.

    Jonker, N., Kool, J., Irth, H. & Niessen, W. M. Anal. Bioanal. Chem. 399, 2669–2681 (2011).

  11. 11.

    Shih, T. C. et al. Mol. Cancer Ther. 16, 1212–1223 (2017).

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The author declares no competing interests.

Correspondence to Kit S. Lam.

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Fig. 1: Affinity selection–mass spectrometry (AS–MS) method for identifying high-affinity peptide ligands.