Single-molecule techniques combined with molecular dynamics simulations allowed visualization of a surprisingly high level of conformational homogeneity in the transport cycle of an ABC import system, BtuCD-F, and revealed an unexpected tight coupling of distinct conformational states responsible for vitamin B12 binding, transport and release.
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Schmitt, L. Vitamin B12 import is all about timing. Nat Chem Biol 14, 640–641 (2018). https://doi.org/10.1038/s41589-018-0087-3