Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

TRANSLATION

Tracking tRNA packages

Fast, single-molecule tracking microscopy monitors transitions between mobile and ribosome-bound fluorescent tRNAs to achieve nucleotide-resolution measurements of translation rates in living cells.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Fluorescently labeled tRNA molecules are delivered into cells via electroporation.

References

  1. Volkov, I. L. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-018-0063-y (2018).

    Article  PubMed  PubMed Central  Google Scholar 

  2. Yu, J., Xiao, J., Ren, X., Lao, K. & Xie, X. S. Science 311, 1600–1603 (2006).

    Article  CAS  PubMed  Google Scholar 

  3. Golding, I. & Cox, E. C. Proc. Natl. Acad. Sci. USA 101, 11310–11315 (2004).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Crawford, R. et al. Biophys. J. 105, 2439–2450 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Plochowietz, A., Farrell, I., Smilansky, Z., Cooperman, B. S. & Kapanidis, A. N. Nucleic Acids Res. 45, 926–937 (2017).

    Article  CAS  PubMed  Google Scholar 

  6. Lindén, M., Ćurić, V., Amselem, E. & Elf, J. Nat. Commun. 8, 15115 (2017).

    Article  PubMed  PubMed Central  Google Scholar 

  7. Rodnina, M. V. Protein Sci. 25, 1390–1406 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Mustafi, M. & Weisshaar, J. C. MBio 9, e02143–17 (2018).

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Achillefs N. Kapanidis.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kapanidis, A.N., Stracy, M. Tracking tRNA packages. Nat Chem Biol 14, 528–529 (2018). https://doi.org/10.1038/s41589-018-0066-8

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41589-018-0066-8

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing