PROTEIN EVOLUTION

Enzymes emerge by upcycling

Catalytic activity is a critical protein function, but its natural evolution has never been observed. Researchers have now revealed the mutations and biochemical mechanisms by which two ancient nonenzymatic proteins acquired catalytic activity.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: Enzyme activity evolved by activating existing residues and quenching dynamics.

References

  1. 1.

    Clifton, B. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-018-0043-2 (2018).

  2. 2.

    Kaltenbach, M. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-018-0042-3 (2018).

  3. 3.

    Mak, W. S. & Siegel, J. B. Curr. Opin. Struct. Biol. 27, 87–94 (2014).

    Article  CAS  Google Scholar 

  4. 4.

    Pauling, L. & Zuckerkandl, E. Acta Chem. Scand. 17, S9–S16 (1963).

    Article  CAS  Google Scholar 

  5. 5.

    Harms, M. J. & Thornton, J. W. Curr. Opin. Struct. Biol. 20, 360–366 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Steindel, P. A., Chen, E. H., Wirth, J. D. & Theobald, D. L. Protein Sci. 25, 1319–1331 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. 7.

    Anderson, D. P. et al. eLife 5, e10147 (2016).

    Article  Google Scholar 

  8. 8.

    Lynch, V. J., May, G. & Wagner, G. P. Nature 480, 383–386 (2011).

    Article  CAS  Google Scholar 

  9. 9.

    Natarajan, C. et al. Science 354, 336–339 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. 10.

    Tzul, F. O., Vasilchuk, D. & Makhatadze, G. I. Proc. Natl. Acad. Sci. USA 114, E1627–E1632 (2017).

    Article  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Michael J. Harms.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Harms, M.J. Enzymes emerge by upcycling. Nat Chem Biol 14, 526–527 (2018). https://doi.org/10.1038/s41589-018-0064-x

Download citation

Further reading