Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined. The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse. By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.
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We thank Geniad Pty Ltd for generously providing CC mice and genotypes. This project was supported in part by the WA Diabetes Research Foundation (to G.M.), Program 1037321 (to G.M.) and Project 1069173 (to G.M.) from the National Health and Medical Research Council of Australia and by European Commission FP7 grants MIMOmics (contract 305280 to G.L.) and H2020 projects GlySign (contract 722095 to G.L.), SYSCID (contract #733100 to G.L.) and IMforFuture (contract 721815 to G.L.), as well as by the European Structural and Investment Funds IRI (grant KK.01.2.1.01.0003 to G.L.) and Croatian National Centre of Research Excellence in Personalized Healthcare (grant KK.01.1.1.01.0010 to G.L.).
G.L. is founder and owner of Genos Ltd, which specializes in high-throughput glycomics analysis and has several patents in the field. G.M. holds stock in Geniad Pty Ltd.
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Figures 1–9 and Supplementary Tables 1–8
List of candidate genes involved in the regulation of mouse IgG glycosylation identified from the QTL analysis.
Strain, sex and age of mice.
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Krištić, J., Zaytseva, O.O., Ram, R. et al. Profiling and genetic control of the murine immunoglobulin G glycome. Nat Chem Biol 14, 516–524 (2018). https://doi.org/10.1038/s41589-018-0034-3
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