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Transposition enables low-input single-molecule concurrent genomics and epigenomics

We adapted Tn5 transposition to reduce the amount of input DNA required for amplification-free single-molecule real-time Pacific Biosciences sequencing. We applied transposition-based library preparation in two sensitive methods for concurrently resolving genomes and epigenomes using limiting amounts of native DNA.

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Fig. 1: Transposition-based single-molecule whole-genome and epigenome profiling in rare cell types.

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This is a summary of: Nanda, A. S. et al. Direct transposition of native DNA for sensitive multimodal single-molecule sequencing. Nat. Genet. https://doi.org/10.1038/s41588-024-01748-0 (2024).

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Transposition enables low-input single-molecule concurrent genomics and epigenomics. Nat Genet 56, 1055–1056 (2024). https://doi.org/10.1038/s41588-024-01753-3

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