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Disentangling extrachromosomal circular DNA heterogeneity in single cells with scEC&T-seq

We introduce scEC&T-seq, a new single-cell sequencing method that enables parallel profiling of extrachromosomal circular DNA and mRNAs in single cells. Using scEC&T-seq, we characterized all types of circular DNA elements in single human cancer cells and profiled the intercellular heterogeneity and structural dynamics of cancer-specific extrachromosomal DNA.

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Fig. 1: scEC&T-seq enables parallel detection of circular DNA and transcriptome in single cells.


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This is a summary of: González, R. C. et al. Parallel sequencing of extrachromosomal circular DNAs and transcriptomes in single cancer cells. Nat. Genet. (2023).

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Disentangling extrachromosomal circular DNA heterogeneity in single cells with scEC&T-seq. Nat Genet 55, 740–741 (2023).

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