A high-throughput reporter gene assay for use in living tissues has been developed. The assay allows investigators to quantify, in parallel, the activities of reporter genes in each of the cell types that constitute a tissue.
This is a preview of subscription content, access via your institution
Access options
Subscribe to Nature+
Get immediate online access to Nature and 55 other Nature journal
$29.99
monthly
Subscribe to Journal
Get full journal access for 1 year
$79.00
only $6.58 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Buy article
Get time limited or full article access on ReadCube.
$32.00
All prices are NET prices.
References
White, M. A. Understanding how cis-regulatory function is encoded in DNA sequence using massively parallel reporter assays and designed sequences. Genomics 106, 165–170 (2015). A review article that describes MPRAs and their use in studying CRSs.
Zhang, F. & Lupski, J. R. Non-coding genetic variants in human disease. Hum. Mol. Genet. 24, 102–110 (2015). A review on the role of non-coding variants in human disease.
Cao, J. et al. Comprehensive single-cell transcriptional profiling of a multicellular organism. Science 357, 661–667 (2017). An article that describes the use of combinatorial indexing to sequence the transcriptomes of the cells in Caenorhabditis elegans.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Zhao, S. et al. A single-cell massively parallel reporter assay detects cell-type-specific gene regulation Nat. Genet. https://doi.org/10.1038/s41588-022-01278-7 (2022).
Rights and permissions
About this article
Cite this article
A scalable technology for measuring cell-type-specific activity of cis-regulatory sequences. Nat Genet (2023). https://doi.org/10.1038/s41588-022-01277-8
Published:
DOI: https://doi.org/10.1038/s41588-022-01277-8
