Current methods of chromatin analysis focus mainly on the most abundant cell types in a sample. We present a workflow that combines enrichment of rare cell types with high-resolution mapping of histone modifications, which enables us to study chromatin dynamics in rare stem and progenitor cell populations.
This is a preview of subscription content, access via your institution
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 per month
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Rent or buy this article
Get just this article for as long as you need it
Prices may be subject to local taxes which are calculated during checkout
Jambhekar, A. et al. Roles and regulation of histone methylation in animal development. Nat. Rev. Mol. Cell Biol. 20, 625–641 (2019). This review summarizes the roles of histone modifications discovered through knockout experiments.
Schmid, M. et al. ChIC and ChEC: Genomic mapping of chromatin proteins. Mol. Cell 16, 147–157 (2004). This paper reports the development of chromatin immunocleavage for antibody-based chromatin analysis by targeted fragmentation.
Baron, C. S., Barve, A. & Muraro, M. J. Cell type purification by single-cell transcriptome-trained sorting. Cell 179, 527–542 (2019). This article presents how integrating single-cell transcriptomics data with FACS index sorting data can be used to set non-intuitive sorting gates for cell-type enrichment in the absence of surface markers or transgenes.
Stoeckius, M. et al. Simultaneous epitope and transcriptome measurement in single cells. Nat. Methods 14, 865–868 (2017). This article presents quantitative proteome analysis by single-cell sequencing.
Skene, P. et al. An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. eLife 6, e21856 (2017). This article presents CUT&RUN as a high-sensitivity sequencing approach for chromatin analysis.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Zeller, P. et al. Single-cell sortChIC identifies hierarchical chromatin dynamics during hematopoiesis. Nat. Genet. https://doi.org/10.1038/s41588-022-01260-3 (2022).
Rights and permissions
About this article
Cite this article
sortChIC enables detailed chromatin analysis of rare cell types. Nat Genet 55, 170–171 (2023). https://doi.org/10.1038/s41588-022-01259-w