Systematic CRISPR screens in primary human T cells uncovered the upstream regulators of crucial immune genes — IL2RA, IL2 and CTLA4. Then, using RNA-seq and ATAC-seq in knockout T cells, we mapped the downstream target genes and non-coding cis-regulatory elements of key regulators, thereby revealing a regulatory network enriched for immune disease-associated genes.
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References
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This is a summary of: Freimer, J. W. et al. Systematic discovery and perturbation of regulatory genes in human T cells reveals the architecture of immune networks. Nat. Genet. https://doi.org/10.1038/s41588-022-01106-y (2022).
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Mapping the regulators of key immune genes and disease networks. Nat Genet 54, 1070–1071 (2022). https://doi.org/10.1038/s41588-022-01107-x
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DOI: https://doi.org/10.1038/s41588-022-01107-x
