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EPIGENOMICS

Histone gene editing probes functions of H3K27 modifications in mammals

A new study employs CRISPR–Cas9-based base editing for simultaneous mutagenesis of all copies of histone H3 genes in mammals, highlighting the functional importance of H3K27me3 for Polycomb-mediated gene silencing and the dispensability of H3K27ac in transcriptional activation.

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Fig. 1: Functional investigation of H3K27 by massive parallel histone mutagenesis.

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Correspondence to Roberto Bonasio.

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Scacchetti, A., Bonasio, R. Histone gene editing probes functions of H3K27 modifications in mammals. Nat Genet 54, 746–747 (2022). https://doi.org/10.1038/s41588-022-01096-x

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