a, Tumors derived from parental PC9 or PC9-Cas9 cells (n = 4 per group). b, Mice from early and late tumor time points (n = 4 and 10 tumors, respectively). c, pgRNA Illumina libraries. d, Pearson correlation (r) matrix for xenograft screen samples. Unsupervised clustering of library depth-normalized pgRNA counts by the complete-linkage method. e, Normalized counts (mean ± S.D.) for gRNAs targeting coding exons in the indicated genes. Data from Chen et al, 2015 (n = 1, 6, 3, and 9 for groups, left to right). f, Relative cell number (mean ± S.D.) for PC9-Cas9 cells expressing a pgRNA targeting the indicating exons (n = 3 per group). g, Progression-free survival of lung adenocarcinoma patients (n = 167/171 for low/high categories), where patients were stratified by inclusion of tumor-suppressive poison exons. h, As (g), but for overall survival. i, As (g), but for essential poison exons (n = 166/169 for low/high categories). j, As (i), but for overall survival. See Source Data for uncropped gels.