(a) Number of TADs in each stage of differentiation as defined by a domain caller algorithm first reported by Dixon et al. 20125. (b) Number of TADs (or loop domains) found by Arrowhead algorithm10. (c) Number of TADs found by Insulation score29. (d) Fraction of TAD boundaries that contain CTCF ChIP-seq peaks at each stage of cardiomyocyte differentiation. ‘stable’ group stands for TADs that are present at all stages. (e) Boxplot showing the dynamics of H3K27ac signal at ESC(+) TADs (N=198). (f) Boxplot showing the dynamics of RNA-seq signal at ESC(+) TADs (N=198). The elements of the boxplot are: center line, median; box limit, upper and lower quartiles; whiskers, 1.5x interquartile range.