Supplementary Fig. 10 | Nature Genetics

Supplementary Fig. 10

From: SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development

Supplementary Fig. 10

a, Bright fields of mouse embryos isolated at E2.5 for control (morula stage) and Setd2mNull embryos (arrested at 1-cell stage). One representative result of three independent experiments is shown. Scale bars: 20 μm. b, Hoechest staining of control (2-cell) and Setd2mNull embryos (arrested at 1-cell prior to pronuclei fusion) obtained at E1.5. One representative result of two independent experiments is shown. Scale bars: 20 μm. c, Bright fields of mouse embryos isolated at E3.5 for control (blastocyst stage) and Setd2f/f;Zp3-Cre embryos. One representative result of two independent experiments is shown. Scale bars: 20 μm. d, EU staining in control and Setd2mNull zygotes at E0.5 (1-cell stage). Dashed lines marked maternal and paternal pronuclei. One representative result of three independent experiments is shown. e, EU signal intensity was calculated by immunofluorescence intensity of maternal/paternal pronuclei against background in control (n = 9) and Setd2mNull zygotes (n = 10) . IF intensity of individual pronuclei from three independent experiments are analyzed. Dots represent single pronuclei, with medians indicated by horizontal lines. Error bar represents s.e.m.. f, Hierarchical clustering analysis of global gene expression in oocytes and early embryos for control (blue) and Setd2mNull mutants (red). g, Heat map showing gene expression in control and Setd2mNull oocytes and embryos.

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