Supplementary Fig. 4: Aberrant H3K4me3 and H3K27me3 in Setd2mNull FGOs. | Nature Genetics

Supplementary Fig. 4: Aberrant H3K4me3 and H3K27me3 in Setd2mNull FGOs.

From: SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development

Supplementary Fig. 4

a, Pie charts demonstrating proportions of the genome covered by H3K4me3, H3K27me3, bivalent marks (H3K4me3 and H3K27me3), H3K36me3, or none of these marks in control and Setd2mNull FGO (Methods). b, A snapshot of UCSC browser showing H3K4me3, H3K27me3 and H3K36me3 enrichment in 2-3 replicates of control and Setd2mNull FGO. Regions that acquire/lost H3K4me3 or acquire H3K27me3 are shaded. c, Box plots showing enrichment of H3K4me3 and H3K27me3 for regions defined as ‘K4me3-gain domains’, ‘K4me3-loss domains’, ‘K27me3-gain domains’, and ‘K27me3-loss domains’ in individual replicates of control (H3K4me3, n = 3; H3K27me3, n = 2) and Setd2mNull FGO (H3K4me3, n = 3; H3K27me3, n = 2). The median of each dataset is shown by center line. The bottom, top edges and whiskers represent the 25th, 75th percentiles and 1.5 times the interquartile range, respectively. d, Box plots showing enrichment of H3K4me3 and H3K27me3 in control (H3K4me3, n = 3; H3K27me3, n = 2) and Setd2mNull FGO (H3K4me3, n = 3; H3K27me3, n = 2) for regions defined as ‘K4me3-gain domains’, ‘K4me3-loss domains’, ‘K27me3-gain domains’, and ‘K27me3-loss domains’. Enrichment of whole genome is shown as controls. The median of each dataset is shown by center line. The bottom, top edges and whiskers represent the 25th, 75th percentiles and 1.5 times the interquartile range, respectively. P-values (t-test, two sided) are also shown.

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