a, Schematic showing the sgRNA target and PAM site, which lies near the intron 1–exon 2 junction of MAGOH. b, Sanger sequencing traces around the MAGOH sgRNA cut site, from single-cell Heya8 clones derived from cells transduced with either non-targeting guide (sgGFP) or guide against MAGOH. In both MAGOH-deleted clones (clones 1 and 5), an indel at the indicated position (black underline) occurs in a similar location and could cause a frameshift. In clone 5, mixed peaks may suggest distinct editing events on each allele. Ablation of MAGOH expression in these clones is shown in Supplementary Fig. 5.