a, Maternal HFD did not significantly affect the average number of ovulated eggs in mice, although there was a slight reduction (n = 8 for ND mice; n = 8 for HFD mice). b, Average litter size was significantly reduced in HFD mice compared to ND mice when these mice were mated with normal males (n = 8 for ND mice; n = 8 for HFD mice). Student’s t test (two-tailed) was used for statistical analysis. Error bars, s.d.; center values, mean; n.s., not significant.