Supplementary Figure 8: MYC-bound enhancer axes reflect tumor-specific pathways in deregulated-MYC-driven cancers. | Nature Genetics

Supplementary Figure 8: MYC-bound enhancer axes reflect tumor-specific pathways in deregulated-MYC-driven cancers.

From: Enhancer invasion shapes MYCN-dependent transcriptional amplification in neuroblastoma

Supplementary Figure 8

a, Top, plot showing the top 5,000 genes (x axis) in SHEP-21N ranked by total proximal MYCN signal. Bottom, dot plot of the percentage of enhancer contribution (enhancer/total MYC signal) sampled across bins (100 genes/bin) with a best fit line superimposed (loess correlation). b, Differential MYC signal contribution in the H2171 cell line for promoters (red) and enhancers (blue) of associated genes (y axis) of the top 5,000 proximal MYC-bound regions are shown ranked by difference in MYC enhancer to promoter contribution (x axis). c, GSEA plots of MYC-bound promoter- (red) versus enhancer- (blue) dominant gene sets defined by leading edge analysis. d, Normalized enrichment scores (NESs) of target gene signatures (Molecular Signature Database) are plotted on the x axis versus the FDR (false discovery rate) on the y axis. e, Highly significant gene signatures from promoter (red) and enhancer (blue) bias gene sets are highlighted and tabulated. fi, Same as in ae (respectively) in the U87 cell line. ko, Same as in ae (respectively) in the MM1.S cell line. pt, Same as in ae (respectively) in the P493-6 cell line.

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