Supplementary Figure 7: Enhancer invasion accounts for tumor-specific MYCN signatures in neuroblastoma. | Nature Genetics

Supplementary Figure 7: Enhancer invasion accounts for tumor-specific MYCN signatures in neuroblastoma.

From: Enhancer invasion shapes MYCN-dependent transcriptional amplification in neuroblastoma

Supplementary Figure 7

a, Differential MYCN signal contribution in the BE(2)-C cell line for promoters (red) and enhancers (blue) of associated genes (y axis) of the top 5,000 proximal MYCN-bound regions are shown ranked by difference in MYCN enhancer to promoter contribution (x axis). b, GSEA plots of MYCN-bound promoter- (red) versus enhancer- (blue) dominant gene sets defined by leading edge analysis. c, Normalized enrichment scores (NESs) of target gene signatures (Molecular Signature Database) are plotted on the x axis versus the FDR (false discovery rate) on the y axis. d, Highly significant gene signatures from promoter (red) and enhancer (blue) bias gene sets are highlighted and tabulated. e, Differential H3K27ac signal contribution in the BE(2)-C cell line for promoters (red) and enhancers (blue) of associated genes (y axis) of the top 5,000 proximal H3K27ac-bound regions are shown ranked by difference in H3K27ac enhancer to promoter contribution (x axis). f, GSEA plots of H3K27ac-bound promoter- (red) versus enhancer- (blue) dominant gene sets defined by leading edge analysis. g, Normalized enrichment scores (NESs) of target gene signatures (Molecular Signature Database) are plotted on the x axis versus the FDR (false discovery rate) on the y axis. h, Highly significant gene signatures from promoter (red) and enhancer (blue) bias gene sets are highlighted and tabulated. i, Overall survival of patients ranked by expression of the top 25 genes defined by total MYCN load at promoter-dominant genes (left) or enhancer-dominant genes (right) for all patients with neuroblastoma. Significance is denoted by a chi-squared test, and P values are shown. j, Overall survival of patients ranked by expression of the top 25 genes defined by total MYCN load at promoter-dominant genes (left) or enhancer-dominant genes (right) for patients with neuroblastoma without MYCN amplification. Significance is denoted by a chi-squared test, and P values are shown. k, Heatmap of gene signatures enriched in promoter- (red) or enhancer- (blue) dominant pathways as defined by MYCN or H3K27ac in the BE(2)-C cell line. Selected signatures are annotated at an FDR < 0.01 and NES > 2 cutoff. l, Normalized enrichment scores (NESs) of target gene signatures (Molecular Signature Database) are plotted on the x axis versus the FDR (false discovery rate) on the y axis at 0, 2, and 6 h after MYCN induction.

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