Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Genetic engineering

Phage genome engineering with retrons

A method using retron recombineering for phage genome modification may facilitate access to therapeutic phages.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Phage retron recombineering.

References

  1. Eskenazi, A. et al. Nat. Commun. 13, 302 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Gencay, Y. E. et al. Nat. Biotechnol. 42, 265–274 (2024).

    Article  CAS  PubMed  Google Scholar 

  3. Dedrick, R. M. et al. Nat. Med. 25, 730–733 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Fishman, C. B. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02370-5 (2024).

    Article  PubMed  Google Scholar 

  5. Mahler, M., Costa, A. R., van Beljouw, S. P. B., Fineran, P. C. & Brouns, S. J. J. Trends Biotechnol. 41, 669–685 (2023).

    Article  CAS  PubMed  Google Scholar 

  6. Millman, A. et al. Cell 183, 1551–1561.e12 (2020).

    Article  CAS  PubMed  Google Scholar 

  7. Yee, T., Furuichi, T., Inouye, S. & Inouye, M. Cell 38, 203–209 (1984).

    Article  CAS  PubMed  Google Scholar 

  8. Simon, A. J., Ellington, A. D. & Finkelstein, I. J. Nucleic Acids Res. 47, 11007–11019 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Doron, S. et al. Science 359, eaar4120 (2018).

    Article  PubMed  PubMed Central  Google Scholar 

  10. Georjon, H. & Bernheim, A. Nat. Rev. Microbiol. 21, 686–700 (2023).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Rotem Sorek.

Ethics declarations

Competing interests

R.S. is a scientific cofounder and advisor of BiomX and Ecophage. I.O. declares no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Osterman, I., Sorek, R. Phage genome engineering with retrons. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02392-z

Download citation

  • Published:

  • DOI: https://doi.org/10.1038/s41587-024-02392-z

Search

Quick links

Nature Briefing Microbiology

Sign up for the Nature Briefing: Microbiology newsletter — what matters in microbiology research, free to your inbox weekly.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing: Microbiology