Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Gene editing

Genome editing with DNA-dependent polymerases

Nature Biotechnology (2024)Cite this article

Subjects

Two CRISPR tools harness DNA-dependent polymerases for genome editing — and may overcome some limitations of prime editors.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Schematic of prime editors (PEs), DNA polymerase editors (DPEs) and click editors (CEs).

References

  1. Anzalone, A. V. et al. Nature 576, 149–157 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Liu, B. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01947-w (2024).

    Article  PubMed  Google Scholar 

  3. Ferreira da Silva, J. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02324-x (2024).

    Article  PubMed  Google Scholar 

  4. Chen, P. J. & Liu, D. R. Nat. Rev. Genet. 24, 161–177 (2023).

    Article  CAS  PubMed  Google Scholar 

  5. Nelson, J. W. et al. Nat. Biotechnol. 40, 402–410 (2022).

    Article  CAS  PubMed  Google Scholar 

  6. Liu, B. et al. Nat. Biotechnol. 40, 1388–1393 (2022).

    Article  CAS  PubMed  Google Scholar 

  7. Feng, Y. et al. Protein Cell https://doi.org/10.1093/procel/pwac014 (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  8. Yan, J. et al. Nature 628, 639–647 (2024).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Anzalone, A. V. et al. Nat. Biotechnol. 40, 731–740 (2022).

    Article  CAS  PubMed  Google Scholar 

  10. Yarnall, M. T. N. et al. Nat. Biotechnol. 41, 500–512 (2023).

    Article  CAS  PubMed  Google Scholar 

  11. Levesque, S., Cosentino, A., Verma, A., Genovese, P. & Bauer, D. E. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02266-4 (2024).

    Article  PubMed  Google Scholar 

  12. Wang, X. et al. Curr. Opin. Biomed. Eng. 28, 100491 (2023).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA

    Quinn T. Cowan & Alexis C. Komor

Authors
  1. Quinn T. Cowan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alexis C. Komor
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alexis C. Komor.

Ethics declarations

Competing interests

A.C.K. is a member of the scientific advisory board of Pairwise Plants, is an equity holder in Pairwise Plants and Beam Therapeutics, and receives royalties from Pairwise Plants, Beam Therapeutics and Editas Medicine via patents licensed from Harvard University. A.C.K.’s interests have been reviewed and approved by the University of California, San Diego in accordance with its conflict of interest policies. Q.T.C. declares no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Cowan, Q.T., Komor, A.C. Genome editing with DNA-dependent polymerases. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02372-3

Download citation

  • Published:

  • DOI: https://doi.org/10.1038/s41587-024-02372-3

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing