Satellite cells, the stem cells of skeletal muscle, are responsible for muscle development and regeneration. Although low in abundance, satellite cells can be isolated from muscle but cannot be propagated successfully in culture in numbers needed for therapeutic use. We developed a method to generate cells with satellite cell characteristics from skeletal muscle organoid cultures.
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References
Mauro, A. Satellite cell of skeletal muscle fibers. J. Biophys. Biochem. Cytol. 9, 493–495 (1961). This paper identified the satellite cell and postulated its role as a stem cell that is responsible for skeletal muscle regeneration.
Montarras, D. & Buckingham, M. Direct isolation of satellite cells for skeletal muscle regeneration. Science 309, 2064–2067 (2005). This paper stands as a great example of how satellite cells lose their stem cell function following traditional culture in vitro.
Judson, R. N. & Rossi, F. M. V. Towards stem cell therapies for skeletal muscle repair. npj Regen Med. 5, 10 (2020). This review provides a summary of recent progress in the field of skeletal muscle cell therapy.
Seale, P. & Rudnicki, M. A. Pax7 is required for the specification of myogenic satellite cells. Cell. 102, 777–786 (2000). This paper identified PAX7 as a key transcription factor that is present in adult satellite cells.
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This is a summary of: Price, F. D. et al. Organoid culture promotes dedifferentiation of mouse myoblasts into stem cells capable of complete muscle regeneration. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02344-7 (2024).
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Dedifferentiating myoblasts back into a satellite cell state in vitro. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02345-6
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DOI: https://doi.org/10.1038/s41587-024-02345-6
