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Synthetic biology

Enzymatic synthesis of RNA oligonucleotides

Nature Biotechnology (2024)Cite this article

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Research on enzymatic RNA synthesis has long been eclipsed by work on DNA—but a new method provides a leap forward for RNA.

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Fig. 1: Comparison of methods to synthesize natural and modified RNA oligonucleotides.

References

  1. Wiegand, D. J. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02244-w (2024).

    Article  Google Scholar 

  2. Paunovska, K., Loughrey, D. & Dahlman, J. E. Nat. Rev. Genet. 23, 265–280 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Egli, M. & Manoharan, M. Nucleic Acids Res. 51, 2529–2573 (2023).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Boccaletto, P. et al. Nucleic Acids Res. 50, D231–D235 (2022).

    Article  CAS  PubMed  Google Scholar 

  5. Moody, E. R., Obexer, R., Nickl, F., Spiess, R. & Lovelock, S. L. Science 380, 1150–1154 (2023).

    Article  CAS  PubMed  Google Scholar 

  6. Caruthers, M. H. Science 230, 281–285 (1985).

    Article  CAS  PubMed  Google Scholar 

  7. Palluk, S. et al. Nat. Biotechnol. 36, 645–650 (2018).

    Article  CAS  PubMed  Google Scholar 

  8. Verardo, D. et al. Sci. Adv. 9, eadi0263 (2023).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Hoose, A., Vellacott, R., Storch, M., Freemont, P.S. & Ryadnov, M.G. Nat. Rev. Chem. 7, 144–161 (2023).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Pichon, M., Levi-Acobas, F., Kitoun, C. & Hollenstein, M. Chemistry 30, e202400137 (2024).

    Article  CAS  PubMed  Google Scholar 

  11. Ruparel, H. et al. Proc. Natl Acad. Sci. USA 102, 5932–5937 (2005).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Institut Pasteur, Université Paris Cité, CNRS UMR 3523, Department of Structural Biology and Chemistry, Laboratory for Bioorganic Chemistry of Nucleic Acids, Paris, France

    Marcel Hollenstein

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  1. Marcel Hollenstein
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Correspondence to Marcel Hollenstein.

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Hollenstein, M. Enzymatic synthesis of RNA oligonucleotides. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02322-z

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